Rabbit Anti-APPL1 antibody | Sunlong Medical

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Adapter protein containing PH domain; Adaptor Protein Containing pH Domain; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1; Adaptor protein containing pH domain PTB domain and leucine zipper motif; AKT2 INTERACTOR; APPL 1; APPL

Cat:

SL1632R

Species Reactivity：

Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human APPL1:101-200/709

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-APPL1 Polyclonal Antibody, Unconjugated(SL1632R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]

Function:
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.

Subunit:
Binds RAB5A/Rab5 through an N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Interacts with OCRL and INPP5B.

Subcellular Location:
Early endosome membrane; Peripheral membrane protein. Nucleus. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.

Tissue Specificity:
High levels in heart, ovary, pancreas and skeletal muscle.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosophorylation at Ser-410 by PKA severely impairs binding to OCRL.

Similarity:
Contains 1 PH domain.
Contains 1 PID domain.

SWISS:
Q9UKG1

Gene ID:
26060

Database links:

Entrez Gene: 26060 Human

Entrez Gene: 72993 Mouse

Entrez Gene: 290537 Rat

Omim: 604299 Human

SwissProt: Q9UKG1 Human

SwissProt: Q8K3H0 Mouse

Unigene: 476415 Human

Unigene: 202322 Mouse

衔接因子蛋白含pH域磷酸酪氨酸结合域和亮氨酸拉链基元1抗体.
APPL1可调节脂联素和胰岛素信号，是细胞膜与细胞核之间重要的信息传递蛋白,同时也是细胞增殖所必需的蛋白，它有调节脂联素在脂肪酸氧化和葡萄糖吸收中的作用，APPL1通过激酶通道来调节脂联素的胰岛素敏感效应。 Picture

Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-APPL1 Polyclonal Antibody, Unconjugated(SL1632R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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