Rabbit Anti-Calnexin antibody

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Calnexin-ER membrane marker; CANX; Cell adhesion molecule with homology to l1cam precursor; CNX; D11Ertd153e; 1110069N15Rik; AI988026; FLJ26570; IP90; P90; Similar to calnexin; Major histocompatibility complex class I antigen-binding protein p88; CALX_HUM

Cat:

SL1693R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CANX:501-592/592

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Blank control:H9C2 Cells(blue). Primary Antibody: Rabbit Anti-CANX/FITC Conjugated antibody (SL1693R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.ProtocolThe cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (SL1693R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Function:
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

Subunit:
Interacts with MAPK3/ERK1. Interacts with KCNH2. Associates with ribosomes. Interacts with SGIP1; involved in negative regulation of endocytosis (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins.

Subcellular Location:
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity). br>Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.

Similarity:
Belongs to the calreticulin family.

SWISS:
P27824

Gene ID:
821

Database links:

Entrez Gene: 821 Human

Entrez Gene: 12330 Mouse

Entrez Gene: 29144 Rat

Entrez Gene: 403908 Dog

Omim: 114217 Human

SwissProt: P24643 Dog

SwissProt: P27824 Human

SwissProt: P35564 Mouse

SwissProt: P35565 Rat

Unigene: 567968 Human

Unigene: 248827 Mouse

Unigene: 1762 Rat

内质网膜蛋白标志物（ER membrane marker）
钙连蛋白 calnexin 存在于内质网中，是内质网膜标志物，属于Ca2+的凝集素样分子伴侣蛋白，可结合蛋白质的寡糖链，防止蛋白质彼此聚集和泛素化，避免折叠不完全的蛋白质离开内质网。 Picture

Blank control:H9C2 Cells(blue).
Primary Antibody: Rabbit Anti-CANX/FITC Conjugated antibody (SL1693R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (SL1693R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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