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Home > Product > Antibody > Rabbit Anti-AMD1/SAMDC antibody
AMD1; S-adenosylmethionine decarboxylase; Adenosylmethionine decarboxylase 1; AdoMetDC; EC 4.1.1.50; FLJ26964; S adenosylmethionine decarboxylase 1; S adenosylmethionine decarboxylase proenzyme; SamDC.
Cat:
SL1887R
Species Reactivity：
Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human SAMDC:51-150/334
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMD1/SAMDC) Polyclonal Antibody, Unconjugated (SL1887R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Datasheet:


The product of this gene is an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low molecular weight aliphatic amines essential for cellular proliferation and tumor promotion. This protein spans 22 kb comprised of 9 exons and 8 introns and encoding two species of mRNA of 2.1 and 3.4-3.6 kb originating from the use of two different polyadenylation signals. The pro protein is an approximate 38.3 kDa which is known to undergo processing at amino acid 68 to yield two fragments of 32 and 6kDa.

Post-translational modifications:
Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the SLCterminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.

Similarity:
Belongs to the eukaryotic AdoMetDC family.

SWISS:
P17707

Gene ID:
262

Database links:

Entrez Gene: 262 Human

Entrez Gene: 11702 Mouse

Entrez Gene: 81128 Rat

Omim: 36196 Human

SwissProt: P17707 Human

SwissProt: P31154 Mouse

SwissProt: P17708 Rat

Unigene: 159118 Human

Unigene: 253533 Mouse

Unigene: 56270 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMD1/SAMDC) Polyclonal Antibody, Unconjugated (SL1887R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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