Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with SL2122R (Anti-GST) at dilution of 1:60,000 incubated at 4℃ overnight.
Sample:
GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 4 ug
Primary: Anti-GST Tag (SL2122R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample:
Lane 1: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug
Lane 2: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug
Primary: Anti-GST tag (SL2122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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