Rabbit Anti-Amphiphysin antibody

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Amphiphysin; Amp; AMPH 1; AMPH1; Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen); Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stiff man

Cat:

SL489R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Amphiphysin:101-200/695

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Amphiphysin Polyclonal Antibody, Unconjugated(SL489R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

Function:
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

Subunit:
Heterodimer with BIN1. Binds SH3GLB1 and AP2A2. Interacts with AP2B1.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.

Tissue Specificity:
Neurons, certain endocrine cell types and spermatocytes.

Similarity:
Contains 1 BAR domain.
Contains 1 SH3 domain.

SWISS:
P49418

Gene ID:
273

Database links:

Entrez Gene: 273 Human

Entrez Gene: 23638 Mouse

Entrez Gene: 60668 Rat

Omim: 600418 Human

SwissProt: P49418 Human

SwissProt: Q7TQF7 Mouse

SwissProt: O08838 Rat

Unigene: 592182 Human

Unigene: 101650 Mouse

Unigene: 44463 Rat

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Amphiphysin Polyclonal Antibody, Unconjugated(SL489R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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