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Home > Product > Antibody > Rabbit Anti-CD44 antibody
LHR; BA-1; CD 44; CD44 antigen; CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and
Cat:
SL2507R
Species Reactivity：
Human,Mouse,(predicted: Rat,Chicken,Pig,Cow,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from rat CD44v6:325-429/429<Cytoplasmic>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000Flow-Cyt=1μg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Blank control: HELA. Primary Antibody (green line): Rabbit Anti-CD44 antibody (SL2507R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control (blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4 °C). Primary Antibody:Rabbit Anti-CD44 antibody (SL2507R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Product PDFs
Datasheet:


CD44 is the receptor for hyaluronic acid (HA). It mediates cell to cell and cell matrix interactions through its affinity for HA. Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. High protein heterogeneity due to several alternative splicing and post translational modification events. CD44R represents a heterogeneous group of CD44 variant isoforms expressed constitutively in epithelial cells and monocyte lineage cells and can be upregulated in activated leukocytes. CD44R, and in particular CD44v6 containing isoforms, may be involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to sites of inflammation. CD44v6 containing isoforms may be involved in tumor metastasis.

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq].

Function:
Main cell surface receptor for hyaluronate. Adhesion to ucosal high endothelial venule and to types I and VI collagen. robably involved in matrix adhesion, lymphocyte activation and ymph node homing.

Subunit:
Interacts with PKN2.

Subcellular Location:
Membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges.

Post-translational modifications:
N-glycosylated.
O-glycosylated; contains chondroitin sulfate glycans which an be more or less sulfated.
Phosphorylated; activation of PKC results in the ephosphorylation of Ser-467 (constitutive phosphorylation site), nd the phosphorylation of Ser-433.

Similarity:
Contains 1 Link domain.

SWISS:
P16070

Gene ID:
960

Database links:

Entrez Gene: 960 Human

Entrez Gene: 12505 Mouse

Entrez Gene: 25406 Rat

Omim: 107269 Human

SwissProt: P16070 Human

SwissProt: P15379 Mouse

SwissProt: P26051 Rat

Unigene: 502328 Human

Unigene: 423621 Mouse

Unigene: 1120 Rat



CD44V6参与细胞与细胞、细胞与基质问的黏附,与细胞骨架蛋白结合，并与细胞的迁移运动有关,与肿瘤细胞的浸润与转移有关。 Picture

Blank control: HELA.
Primary Antibody (green line): Rabbit Anti-CD44 antibody (SL2507R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4 °C).
Primary Antibody:Rabbit Anti-CD44 antibody (SL2507R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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