Rabbit Anti-Granzyme A antibody

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CTL tryptase; CTLA3; Cytolytic t cell and natural killer cell specific trypsin like serine protease; Cytotoxic T lymphocyte associated serine esterase 3; Cytotoxic T lymphocyte proteinase 1; Cytotoxic T-lymphocyte proteinase 1; Fragmentin 1; Fragmentin-1;

Cat:

SL2578R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human GZMA:101-200/262

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti- Granzyme A Polyclonal Antibody, Unconjugated(SL2578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

Function:
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis.

Subunit:
Homodimer; disulfide-linked. Interacts with APEX1.

Subcellular Location:
Isoform alpha: Secreted. Cytoplasmic granule.

Similarity:
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.

SWISS:
P12544

Gene ID:
3001

Database links:

Entrez Gene: 3001 Human

Omim: 140050 Human

SwissProt: P12544 Human

Unigene: 90708 Human

GzmA是存在于细胞毒性T淋巴细胞(CTL)和自然杀伤细胞(NK细胞)的细胞毒颗粒中含量最多的一种丝氨酸蛋白酶。 Picture

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti- Granzyme A Polyclonal Antibody, Unconjugated(SL2578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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