Rabbit Anti-IL27R antibody | Sunlong Medical

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Interleukin-27 receptor subunit alpha; IL27RA; IL-27R; IL27R alpha; IL-27R-alpha; IL-27RA; Interleukin 27 receptor alpha chain precursor; Protein CRL1; Type 1 T cell cytokine receptor; WSX1; Interleukin-27 Receptor, T Cell Cytokine Receptor; CRL1; zcytor1

Cat:

SL2711R

Species Reactivity：

Mouse,(predicted: Human,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human IL27RA:21-120/636<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IL-27R/TCCR Polyclonal Antibody, Unconjugated(SL2711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Upon antigen challenge, T-helper cells differentiate into two functionally distinct subsets, Th1 and Th2. Th1 cells produce IL-2, IFN-gamma and lymphotoxin-b that augment the cell-mediated immune response, while Th2 cells secrete IL-4, IL-5, and IL-10 that enhance humoral immunity. The function of T-helper cells is regulated by cytokines. A novel cytokine receptor was recently identified and cloned. It is a new member in the type I cytokine receptor family and designated TCCR for T-cell cytokine receptor and WSX-1. TCCR deficient mice had impaired Th1 responses to protein antigen challenge, including decreased levels of IFN-gamma and Th1-dependent antibody IgG2a. TCCR is predominantly expressed in thymus, spleen, lymph nodes and peripheral blood leukocytes.

Function:
Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes.

Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains.

SWISS:
Q6UWB1

Gene ID:
9466

Database links:

Entrez Gene: 9466 Human

Entrez Gene: 50931 Mouse

Entrez Gene: 288905 Rat

Omim: 605350 Human

SwissProt: Q6UWB1 Human

SwissProt: O70394 Mouse

Unigene: 132781 Human

Unigene: 38386 Mouse

Picture

Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IL-27R/TCCR Polyclonal Antibody, Unconjugated(SL2711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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