Rabbit Anti-Phospho-Ezrin (Thr567) antibody

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Ezrin (phospho T567); Ezrin (phospho Thr567); p-Ezrin (phospho T567); CVIL; CVL; cytovillin 2 ; Cytovillin ; DKFZp762H157; FLJ26216; MGC1584; p81; VIL 2 ; VIL2; Villin 2; Villin2; EZRI_HUMAN.

Cat:

SL3134R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human Ezrin around the phosphorylation site of Thr567:YK(p-T)LR

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Small intestine (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-Ezrin (Thr567) (SL3134R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 64 kDObserved band size: 64 kDParaformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (SL3134R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (SL3134R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Function:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Subunit:
Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS.

Subcellular Location:
Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes. Microvillar peripheral membrane protein (cytoplasmic side).

Tissue Specificity:
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Post-translational modifications:
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and SLCterminal halves resulting in an opened conformation which is capable of actin and membrane-bindin.

Similarity:
Contains 1 FERM domain.

SWISS:
P15311

Gene ID:
7430

Database links:

Entrez Gene: 7430 Human

Entrez Gene: 281574 Cow

Entrez Gene: 22350 Mouse

Entrez Gene: 54319 Rat

Omim: 123900 Human

SwissProt: P31976 Cow

SwissProt: P15311 Human

SwissProt: P26040 Mouse

SwissProt: P31977 Rat

Unigene: 487027 Human

Unigene: 277812 Mouse

Unigene: 773 Rat

信号传导（Signaling Intermediates）
目前研究发现Ezrin蛋白与维持细胞的形状、极性、生长运动以及信号转导方面发挥重要作用，该蛋白与肿瘤的侵袭、转移有关。 Picture

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (SL3134R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (SL3134R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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