Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti- Phospho-c-Fos (Ser32) /AF488 antibody(SL3152R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange),used under the same conditions.