This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Function:
Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.
Subunit:
Homodimer. Also forms multiarm oligomers by association of SLCterminal tail domains, oligomers may further assemble to form a hexagonal nuclear lattice-like network. Interacts with TNKS.
Subcellular Location:
Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Note=Resides in the nuclear matrix during interphases. Dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via dynein/dynamin association, it remain there until the anaphase onset. Before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
Isoform Numa-m: Cytoplasm. Note=Mainly clustered at the centrosomal region.
Isoform Numa-s: Cytoplasm. Note=Mainly clustered at the centrosomal region.
Post-translational modifications:
ADP-ribosylated by TNKS during mitosis.
Phosphorylated in the SLCterminal tail during mitosis, probably by CDK1. Phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.
SWISS:
Q14196
Gene ID:
4926
Database links:
Entrez Gene: 4926 Human
Entrez Gene: 101706 Mouse
Entrez Gene: 308870 Rat
Omim: 112809 Human
SwissProt: Q14196 Human
SwissProt: Q3TH77 Mouse
SwissProt: Q4G051 Rat
Unigene: 325978 Human
Unigene: 66392 Rat
NuMA (Nuclear mitotic apparatus protein)是细胞核基质中的一种高分子量蛋白质，具有一个特殊的细胞周期依赖性.核有丝分裂器蛋白NuMA与人类肿瘤细胞的恶性增殖相关.
| Picture |
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NuMA) Polyclonal Antibody, Unconjugated (SL3512R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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