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Home > Product > Antibody > Rabbit Anti-Phospho-Nur77 (Ser351) antibody
NR4A1(phospho S351); NUR77 (phospho S351); p-NUR77 (phospho S351); GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10;
Cat:
SL3313R
Species Reactivity：
Mouse,(predicted: Human,Rat,Chicken,Dog,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated Synthesised phosphopeptide derived from human Nur77 around the phosphorylation site of Ser351:LP(p-S)KP
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Nur77(Ser351)) Polyclonal Antibody, Unconjugated (SL3313R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Product PDFs
Datasheet:


This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Function:
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.

Subunit:
Binds DNA as a monomer. Interacts with GADD45GIP1.

Subcellular Location:
Nucleus.

Tissue Specificity:
Fetal muscle and adult liver, brain and thyroid.

Post-translational modifications:
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.

Similarity:
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P22736

Gene ID:
3164

Database links:

Entrez Gene: 3164 Human

Entrez Gene: 15370 Mouse

Entrez Gene: 7948 Rat

Omim: 139139 Human

SwissProt: P22736 Human

SwissProt: P12813 Mouse

SwissProt: P22829 Rat

Unigene: 524430 Human

Unigene: 119 Mouse

Unigene: 10000 Rat



孤儿核受体蛋白Nur77是早期反应转录因子之一，多种刺激因子如缺氧可诱导其迅速表达，Nur77参与调控细胞增殖、存活、分化等过程。这类核受体是体内广泛分布的配体依赖性的转录因子，通过调控基因协调表达，在机体的生长发育、新陈代谢，细胞分化及体内许多生长发育过程中发挥重要作用，其功能障碍将导致一系列疾病的发生，如；癌症、糖尿病、肥胖、不孕不育等等。 Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Nur77(Ser351)) Polyclonal Antibody, Unconjugated (SL3313R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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