PBK (phospho T9); PBK (phospho Thr9); Cancer/testis antigen 84; CT84; FLJ14385; Lymphokine activated killer T cell originated protein kinase; MAPKK like protein kinase; PDZ-binding-kinase/T-LAK cell-originated protein kinase; Nori 3; Nori3; PDZ binding ki
Cat:
SL3326R
Species Reactivity：
Mouse,(predicted: Human,Rat,)
Immunogen：
KLH conjugated synthesised phosphopeptide derived from human PBK/TOPK around the phosphorylation site of Thr9:FK(p-T)P
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-PBK (Thr9) (SL3326R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kD
This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Subunit: Interacts with DLG1 and TP53.
Tissue Specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Post-translational modifications: Phosphorylated; in a cell-cycle dependent manner at mitosis.
Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
