[email protected]
Home > Product > Antibody > Rabbit Anti-KLF8 antibody
Basic krueppel-like factor 3; BKLF3; DXS741; Kruppel like factor 8; Zinc finger protein 741; ZNF741.
Cat:
SL3649R
Species Reactivity：
Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human KLF8:261-317 /359
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF8) Polyclonal Antibody, Unconjugated (SL3649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


KLF8 is a transcriptional repressor and activator. It also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G phase as a downstream target of focal adhesion kinase (FAK). Ubiquitously expressed.
Kruppel-like factor proteins all contain a characteristic SLCterminal DNA binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers.

Function:
Transcriptional repressor and activator. Binds to CACCSLCboxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).

Subunit:
Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.

Similarity:
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
O95600

Gene ID:
11279

Database links:

Entrez Gene: 11279 Human

Omim: 300286 Human

SwissProt: O95600 Human

Unigene: 646614 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF8) Polyclonal Antibody, Unconjugated (SL3649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.