Rabbit Anti-NDUFA8 antibody

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Complex I 19kD; CI 19KD; CI PGIV; Complex I 19k; Complex I PGIV; MGC793; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8; NA

Cat:

SL3958R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human NDUFA8:101-172/172

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-NDUFA8 Polyclonal Antibody, Unconjugated(SL3958R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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NDUFA8 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Function:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subunit:
Complex I is composed of 45 different subunits.

Subcellular Location:
Mitochondrion. Mitochondrion intermembrane space.

Post-translational modifications:
May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.

Similarity:
Belongs to the complex I NDUFA8 subunit family.
Contains 2 CHCH domains.

SWISS:
P51970

Gene ID:
4702

Database links:

Entrez Gene: 4702 Human

Entrez Gene: 68375 Mouse

Omim: 603359 Human

SwissProt: P51970 Human

SwissProt: Q9DCJ5 Mouse

Unigene: 495039 Human

Unigene: 19834 Mouse

Picture

Tissue/cell: mouse brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NDUFA8 Polyclonal Antibody, Unconjugated(SL3958R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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