Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Human K562 cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human THP-1 cell lysates
Primary: Anti-ACADM (SL4047R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACADM) Polyclonal Antibody, Unconjugated (SL4047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.