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Home > Product > Antibody > Rabbit Anti-EHHADH antibody
3 hydroxyacyl CoA dehydrogenase; 3,2 trans enoyl CoA isomerase; ECHD antibody Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase; L 3 hydroxyacyl CoA dehydrogenase; L bifunctional protein, peroxisomal; L PBE; LBFP; LBP; MGC120586; PBE; PBF
Cat:
SL4058R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human EHHADH:631-723/723
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EHHADH) Polyclonal Antibody, Unconjugated (SL4058R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Unit:
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Product PDFs
Datasheet:


EHHADH is one of three preoxisomal enzymes of the classic peroxisomal Beta-oxidation cycle involved in the catabolism of free fatty acids.Most dietary long chain fatty acids are oxidized in the mitochondria, but very-long-chain fatty acids (VLCFAs) must be shortened first by peroxisomal Beta-oxidation prior to complete oxidation in mitochondria.

Subunit:
Monomer.

Subcellular Location:
Peroxisome.

Tissue Specificity:
Liver and kidney. Lower amounts seen in the brain.

Post-translational modifications:
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.

Similarity:
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the SLCterminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

SWISS:
Q08426

Gene ID:
1962

Database links:

Entrez Gene: 1962 Human

Entrez Gene: 74147 Mouse

Omim: 607037 Human

SwissProt: Q08426 Human

SwissProt: Q9DBM2 Mouse

Unigene: 429879 Human

Unigene: 28100 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EHHADH) Polyclonal Antibody, Unconjugated (SL4058R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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