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Home > Product > Antibody > Rabbit Anti-CAPZA1 antibody
Cap Z; Cappa 1; Cappa1; Capping protein (actin filament) muscle Z line alpha 1; Capping protein alpha 1; Capping protein muscle Z line alpha 1; CapZ alpha 1; CAPZ; CAPZA 1; CAZ 1; CAZ1; F actin capping protein alpha 1 subunit.
Cat:
SL5161R
Species Reactivity：
Human,(predicted: Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human CAPZA1:201-286/286
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPZA1) Polyclonal Antibody, Unconjugated (SL5161R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments, and has roles in cell motility and actin assembly.

Function:
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with S100A (By similarity). Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts with S100B.

Subcellular Location:
Cytoplasm, cytoskeleton (By similarity).

Similarity:
Belongs to the F-actin-capping protein alpha subunit family.

SWISS:
P52907

Gene ID:
829

Database links:

Entrez Gene: 829 Human

Entrez Gene: 12340 Mouse

Entrez Gene: 691149 Rat

Omim: 60316 Human

SwissProt: P52907 Human

SwissProt: P47753 Mouse

SwissProt: B2GUZ5 Rat

Unigene: 727604 Human

Unigene: 19142 Mouse

Unigene: 13838 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPZA1) Polyclonal Antibody, Unconjugated (SL5161R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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