The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, PLCZ1 hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. It triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. PLCZ1 is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. It may exert an inhibitory effect on phospholipase-SLCcoupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Tissue Specificity:
Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
SWISS:
Q86YW0
Gene ID:
89869
Database links:
Entrez Gene: 89869 Human
Entrez Gene: 114875 Mouse
Entrez Gene: 497197 Rat
Omim: 608075 Human
SwissProt: Q86YW0 Human
SwissProt: Q8K4D7 Mouse
SwissProt: Q5FX52 Rat
Unigene: 97542 Human
Unigene: 50808 Mouse
Unigene: 123184 Rat
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Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PLCZ1 Polyclonal Antibody, Unconjugated(SL5378R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (SL5378R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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