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Home > Product > Antibody > Rabbit Anti-phospho-14-3-3 epsilon (Thr232) antibody
YWHAE(phospho T232); 14-3-3 epsilon (phospho Thr232); 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 14-3-3ε; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit;
Cat:
SL5590R
Species Reactivity：
Human,(predicted: Mouse,Rat,Cow,Sheep,)
Immunogen：
KLH conjugated Synthesised phosphopeptide derived from human 14-3-3E around the phosphorylation site of Thr232:LW(p-T)SD
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000Flow-Cyt=1μg/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Blank control (blue line): MCF7,fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 epsilon (Thr232) antibody (SL5590R),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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Unit:
Price: $
Product PDFs
Datasheet:


Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit:
Homodimer. Heterodimerizes with YWHAZ. Interacts with NDEL1, RGNEF and TIAM2. Interacts with HCV core protein. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2.

Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the 14-3-3 family.

SWISS:
P62258

Gene ID:
7531

Database links:

Entrez Gene: 7531 Human

Entrez Gene: 282125 Cow

Entrez Gene: 22627 Mouse

Entrez Gene: 29753 Rat

Omim: 605066 Human

SwissProt: P62261 Cow

SwissProt: P62258 Human

SwissProt: P62259 Mouse

SwissProt: P62260 Rat

Unigene: 513851 Human

Unigene: 234700 Mouse

Unigene: 471625 Mouse

Unigene: 4225 Rat



Picture

Blank control (blue line): MCF7,fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice
Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 epsilon (Thr232) antibody (SL5590R),Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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