The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Function:
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
Subunit:
Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Auto-ISGylated.
Similarity:
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
SWISS:
Q14258
Gene ID:
7706
Database links:
Entrez Gene: 7706 Human
Entrez Gene: 217069 Mouse
Entrez Gene: 494338 Rat
Omim: 600453 Human
SwissProt: Q14258 Human
SwissProt: Q61510 Mouse
Unigene: 528952 Human
Unigene: 248445 Mouse
Unigene: 34382 Rat
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Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti- TRIM25 (SL4170R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 73 kD
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