GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Function:
GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Subunit:
Isoform 1 interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2 (By similarity). Isoform 2 interacts with activated AKT1 in the presence of guanine nucleotides, and with the AP-1 complex.
Subcellular Location:
Isoform 1: Cytoplasm. Nucleus.
Isoform 2: Cytoplasm.
Tissue Specificity:
Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
Post-translational modifications:
Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
Similarity:
Belongs to the centaurin gamma-like family.
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain
SWISS:
Q99490
Gene ID:
116986
Database links:
Entrez Gene: 116986 Human
Entrez Gene: 10524496 Mouse
Entrez Gene: 216439 Mouse
Entrez Gene: 65218 Rat
Omim: 605476 Human
SwissProt: Q99490 Human
SwissProt: Q3UHD9 Mouse
SwissProt: Q8CGU4 Rat
SwissProt: Q9JHW8 Rat
Unigene: 302435 Human
Unigene: 336761 Mouse
Unigene: 91094 Rat
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Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- Centaurin gamma 1 (SL5930R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 125 kD
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