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Home > Product > Antibody > Rabbit Anti-RIL antibody
Enigma homolog; LIM domain protein; LIM protein RIL; PDLIM 4; PDLIM4; PDZ and LIM domain 4; PDZ and LIM domain protein 4; Reversion induced LIM protein; PDLI4_HUMAN.
Cat:
SL6093R
Species Reactivity：
Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human RIL:251-330/330
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RIL) Polyclonal Antibody, Unconjugated (SL6093R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


RIL, also known as PDLIM4 is a putative tumor suppressor gene. RIL is transcriptionally repressed by hypermethylation in a variety of tumors including breast and prostate. The difference in methylation status of RIL between normal and cancerous tissues is being investigated as to determine the use of RIL as a potential biomarker. Two isoforms of human RIL one have been identified.

Subcellular Location:
Cytoplasmic

Similarity:
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.

SWISS:
P50479

Gene ID:
8572

Database links:

Entrez Gene: 8572 Human

Entrez Gene: 30794 Mouse

Entrez Gene: 24915 Rat

Omim: 603422 Human

SwissProt: P50479 Human

SwissProt: P70271 Mouse

SwissProt: P36202 Rat

Unigene: 424312 Human

Unigene: 21830 Mouse

Unigene: 34221 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RIL) Polyclonal Antibody, Unconjugated (SL6093R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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