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Home > Product > Antibody > Rabbit Anti-GRAP2 antibody
Adapter protein GRID; GADS; GADS protein; GRAP-2; GRAP2; GRAP2_HUMAN; GRB-2-like protein; GRB2-related adapter protein 2; GRB2-related adaptor protein 2; GRB2-related protein with insert domain; GRB2L; GRBLG; GrbX; Grf-40; Grf40 adapter protein; Grf40; GR
Cat:
SL6164R
Species Reactivity：
Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human GRAP2:101-200/330
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRAP2) Polyclonal Antibody, Unconjugated (SL6164R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Function:
Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Subunit:
Interacts with phosphorylated LIME1 upon TCR activation (By similarity). Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23.

Subcellular Location:
Nucleus. Cytoplasm. Endosome.

Similarity:
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.

SWISS:
O75791

Gene ID:
9402

Database links:

Entrez Gene: 9402 Human

Entrez Gene: 17444 Mouse

Entrez Gene: 366962 Rat

Omim: 604518 Human

SwissProt: O75791 Human

SwissProt: O89100 Mouse

Unigene: 517499 Human

Unigene: 12947 Mouse

Unigene: 201364 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRAP2) Polyclonal Antibody, Unconjugated (SL6164R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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