Rabbit Anti-ROR beta antibody

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bA133M9.1; NR1F2; Nuclear receptor ROR-beta; Nuclear receptor RZR beta; Nuclear receptor RZR-beta; Nuclear receptor subfamily 1 group F member 2; OTTHUMP00000021486; RAR related orphan receptor B; RAR related orphan receptor beta; Retinoic acid binding re

Cat:

SL6216R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RORB:385-470/470

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:cerebum (Mouse) Lysate at 40 ugPrimary: Anti- ROR beta (SL6216R)at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53 kDObserved band size: 53 kDSample: Eye(Mouse) Lysate at 40 ugEye(Rat) Lysate at 40 ugCerebellum(Mouse) Lysate at 40 ugCerebellum(Rat) Lysate at 40 ugUterus(Mouse) Lysate at 40 ugPrimary: Anti-ROR beta (SL6216R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53 kDObserved band size: 53 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ROR beta) Polyclonal Antibody, Unconjugated (SL6216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ROR beta is a member of the NR1 subfamily of nuclear hormone receptors. It is a DNA-binding protein that can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation.

Function:
Orphan nuclear receptor required for normal postnatal development of rod and cone photoreceptor cells. Regulates transcription of OPN1SW in cone photoreceptor cells by binding the sequence 5'-AGGTCA-3' in the OPN1SW promoter (By similarity).

Subcellular Location:
Nucleus (Probable).

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
Q92753

Gene ID:
6096

Database links:

Entrez Gene: 6096 Human

Entrez Gene: 225998 Mouse

Entrez Gene: 309288 Rat

Omim: 601972 Human

SwissProt: Q92753 Human

SwissProt: Q8R1B8 Mouse

SwissProt: P45446 Rat

Unigene: 494178 Human

Unigene: 234641 Mouse

Unigene: 210157 Rat

Unigene: 231652 Rat

Picture

Sample:
Eye(Mouse) Lysate at 40 ug
Eye(Rat) Lysate at 40 ug
Cerebellum(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Uterus(Mouse) Lysate at 40 ug
Primary: Anti-ROR beta (SL6216R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ROR beta) Polyclonal Antibody, Unconjugated (SL6216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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