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Home > Product > Antibody > Rabbit Anti-OTUB2 antibody
C14orf137; Deubiquitinating enzyme OTUB2; OTB2; OTU domain containing ubiquitin aldehyde binding protein 2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU2; OTUB2_HUMAN; Otubain 2; Otubain-2; Ubiq
Cat:
SL6236R
Species Reactivity：
Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human OTUB:21-110/234
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUB2) Polyclonal Antibody, Unconjugated (SL6236R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.

Function:
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Tissue Specificity:
Widely expressed. Expressed at higher level in brain.

Similarity:
Belongs to the peptidase C65 family.
Contains 1 OTU domain.

SWISS:
Q96DC9

Gene ID:
78990

Database links:

Entrez Gene: 504176 Cow

Entrez Gene: 78990 Human

Entrez Gene: 68149 Mouse

Entrez Gene: 31885 Rat

Omim: 608338 Human

SwissProt: Q96DC9 Human

SwissProt: Q9CQX0 Mouse

Unigene: 278815 Human

Unigene: 127378 Mouse

Unigene: 18862 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUB2) Polyclonal Antibody, Unconjugated (SL6236R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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