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Home > Product > Antibody > Rabbit Anti-UEVLD antibody
ATTP; Ubiquitin-conjugating enzyme E2 variant 3; EV and lactate malate dehydrogenase domain containing protein; EV and lactate/malate dehydrogenase domain-containing protein; signaling molecule ATTP; ubiquitin conjugating enzyme E2 like; Ubiquitin conjuga
Cat:
SL6287R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human UEVLD:85-180/471
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UEVLD) Polyclonal Antibody, Unconjugated (SL6287R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product PDFs
Datasheet:


UEV3, also known as EV and lactate/malate dehydrogenase domain-containing protein, is a 471 amino acid protein that contains one UEV (ubiquitin E2 variant) domain, which typically interacts with ubiquitin. UEV3 is thought to be a paralogue of tsg 101, a protein that exerts regulatory effects on E2 activity in cellular ubiquitination processes. With amino-terminal homology to the catalytic domain of ubiquitin-conjugating enzymes, it is thought that UEV3 may function as a negative regulator of polyubiquitination. UEV3 is expressed in various colon carcinoma cell lines, carcinomas of the uterine cervix and peripheral blood leukocytes as well as normal colon and cervical epithelium.

Subunit:
Homodimer.

Tissue Specificity:
Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.

Similarity:
In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
In the SLCterminal section; belongs to the LDH/MDH superfamily.
Contains 1 UEV (ubiquitin E2 variant) domain.

SWISS:
Q8IX04

Gene ID:
55293

Database links:

Entrez Gene: 55293 Human

Omim: 62205 Human

SwissProt: Q8IX04 Human

Unigene: 407991 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UEVLD) Polyclonal Antibody, Unconjugated (SL6287R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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