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Home > Product > Antibody > Rabbit Anti-RNF45 antibody
AMF receptor; AMF receptor isoform 1; AMF receptor isoform 2; AMFR; AMFR2_HUMAN; Autocrine motility factor receptor; Autocrine motility factor receptor precursor, isoform 1; Autocrine motility factor receptor precursor, isoform 2; E3 ubiquitin-protein lig
Cat:
SL6511R
Species Reactivity：
Rat,(predicted: Human,Mouse,Chicken,Dog,Cow,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human AMFR/RNF45:85-180/643
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF45) Polyclonal Antibody, Unconjugated (SL6511R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product PDFs
Datasheet:


E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.

Subcellular Location:
Endoplasmic reticulum membrane.

Similarity:
Contains 1 CUE domain.
Contains 1 RING-type zinc finger.

SWISS:
Q9UKV5

Gene ID:
267

Database links:

Entrez Gene: 267 Human

Entrez Gene: 4762 Mouse

Omim: 603243 Human

SwissProt: Q9UKV5 Human

SwissProt: Q9R049 Mouse

Unigene: 295137 Human

Unigene: 34641 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF45) Polyclonal Antibody, Unconjugated (SL6511R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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