Rabbit Anti-RBBP6 antibody | Sunlong Medical

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Retinoblastoma binding protein 6; RBBP6; MY038; P2P R; P2PR; p53 associated cellular protein of testis; PACT;Proliferationpotentialrelated protein; Protein P2P R; Protein P2PR; Protein RBQ 1; Protein RBQ1; RB binding Q protein 1; RBBP 6; RBQ 1; RBQ1; Reti

Cat:

SL6576R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RBBP6:1201-1350/1792

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human Placenta; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-RBBP6, Unconjugated(SL6576R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. Retinoblastoma binding protein 6 binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene.

Function:
E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.

Subunit:
Interacts with p53/TP53 and RB1 (By similarity). Interacts also with MDM2 and YBX1. Interacts with NEK6.

Subcellular Location:
ucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.

Tissue Specificity:
Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by NEK6.

Similarity:
Contains 1 CCHSLCtype zinc finger.
Contains 1 DWNN domain.
Contains 1 RING-type zinc finger.

SWISS:
Q7Z6E9

Gene ID:
5930

Database links:

Entrez Gene: 5930 Human

Entrez Gene: 19647 Mouse

Entrez Gene: 308968 Rat

Omim: 600938 Human

SwissProt: Q7Z6E9 Human

SwissProt: P97868 Mouse

Unigene: 188553 Human

Unigene: 496 Mouse

Unigene: 95305 Rat

Picture

Tissue/cell: human Placenta; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-RBBP6, Unconjugated(SL6576R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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