Rabbit Anti-Nucleolar protein 3 antibody

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Apoptosis repressor with CARD; ARC; Muscle enriched cytoplasmic protein; MYC; Myp; NOP; Nop30; Nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein 3; Nucleolar protein of 30 kDa; NOL3_MOUSE .

Cat:

SL7080R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse Nucleolar protein 3/Apoptosis repressor with CARD:1-100/220

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Nucleolar protein 3 Polyclonal Antibody, Unconjugated(SL7080R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD)bcontaining molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf 1, caspase 9 and caspase 2. Apoptosis repressor with CARD is a CARD domain containing protein that interacts with caspase 2 and 8 to inhibit enzymatic activity of caspase 8. Apoptosis repressor with CARD suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR 1, and DR3. The mRNA of Apoptosis repressor with CARD is primarily expressed in skeletal muscle and cardiac tissue. The nuclear isoform (1/Nop30) may be involved in RNA splicing and the cytoplasmic isoform (2/Myp) may inhibit apoptosis.

Function:
May be involved in RNA splicing (By similarity).

Subunit:
Interacts with SFRS9/SRp30c, NPM1, CASP2, CASP8 and TFPT (By similarity).

Subcellular Location:
Cytoplasm and Nucleus > nucleolus.

Similarity:
Contains 1 CARD domain.

SWISS:
O60936

Gene ID:
78688

Database links:

Entrez Gene: 8996 Human

Entrez Gene: 78688 Mouse

Entrez Gene: 85383 Rat

Omim: 605235 Human

SwissProt: O60936 Human

SwissProt: Q9D1X0 Mouse

SwissProt: Q62881 Rat

Unigene: 513667 Human

Unigene: 204876 Mouse

Unigene: 475715 Mouse

Unigene: 86956 Rat

Picture

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Nucleolar protein 3 Polyclonal Antibody, Unconjugated(SL7080R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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