Rabbit Anti-ERN2 antibody | Sunlong Medical

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IRE1b; Endoplasmic reticulum to nucleus signaling 2; ER to nucleus signalling 2; ERN 2; Ern1; hIRE2p; Inositol requiring 1 beta; Inositol requiring protein 2; Ire1; Ire1 beta; IRE1 S. cerevisiae homolog of; IRE2; Serine/threonine protein kinase/endoribonu

Cat:

SL6771R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ERN2:465-550/926

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000Flow-Cyt=2ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-ERN2 antibody (SL6771R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488Dilution: 1μg /test. ProtocolThe cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ERN2, endoplasmic reticulum to nucleus signaling 2, induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. It is pro-apoptotic and appears to play no role in the unfolded-protein response, unlike closely related proteins.

Function:
Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.

SWISS:
O7592

Gene ID:
10595

Database links:

Entrez Gene: 10595 Human

Entrez Gene: 26918 Mouse

Omim: 604034 Human

SwissProt: O7592 Human

SwissProt: Q9EQY0 Mouse

Unigene: 133982 Human

Unigene: 20452 Mouse

Picture

Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-ERN2 antibody (SL6771R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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