Home > Product > Antibody > Rabbit Anti-BOD1 antibody
Biorientation defective 1; Biorientation defective protein 1; biorientation of chromosomes in cell division 1; Biorientation of chromosomes in cell division protein 1; BOD1; BOD1_HUMAN; FAM44B; family with sequence similarity 44, member B; Protein FAM44B.
Cat:
SL6900R
Species Reactivity:
Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Rabbit,)
Immunogen:
KLH conjugated synthetic peptide derived from human BOD1:51-150/185
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BOD1 Polyclonal Antibody, Unconjugated(SL6900R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Unit:
Price: $
Product PDFs
Datasheet:


Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Note=Localizes at the centrosomes throughout the cell cycle, only dissociating during cytokinesis. Localizes at the kinetochore from prometaphase until anaphase.

Similarity:
Belongs to the BOD1 family.

SWISS:
Q96IK1

Gene ID:
91272

Picture

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-BOD1 Polyclonal Antibody, Unconjugated(SL6900R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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