[email protected]
Home > Product > Antibody > Rabbit Anti-NEK1 antibody
NEK1; NEK1_HUMAN; Never in mitosis A-related kinase 1; NIMA (never in mitosis gene a)-related kinase 1; NIMA-related kinase 1; NimA-related protein kinase 1; NY-REN-55; Renal carcinoma antigen NY-REN-55; Serine/threonine-protein kinase Nek1; SRPS2; DKFZp6
Cat:
SL7814R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Cow,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human NEK1:301-400/1258
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (NEK1) Polyclonal Antibody, Unconjugated (SL7814R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


The protein encoded by this gene is a serine/threoninekinase involved in cell cycle regulation. The encoded protein isfound in a centrosomal complex with FEZ1, a neuronal protein thatplays a role in axonal development. Defects in this gene are acause of polycystic kidney disease (PKD). Several transcriptvariants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2010].

Function:
Phosphorylates serines and threonines, but also appearsto possess tyrosine kinase activity. Implicated in the control ofmeiosis (By similarity). Involved in cilium assembly.

Subunit:
Binds to SPERT (By similarity).

Subcellular Location:
Nucleus (Probable). Cytoplasm, cytoskeleton,centrosome (By similarity). Note=Associated with the pericentriolarmaterial. Localizes to centrosome during interphase and mitosis (Bysimilarity).

Tissue Specificity:
High fetal expression in the brain and kidney.

DISEASE:
Defects in NEK1 are the cause of short rib-polydactylysyndrome type 2 (SRPS2) [MIM:263520]. A lethal skeletal dysplasiacharacterized by markedly short ribs, short limbs, polydactyly, andmultiple anomalies including a narrow thorax with hypoplasticlungs, extreme polysyndactyly, dysproportionate dwarfism, mediancleft lip and palate, a ventriculoseptal defect and cystic kidneys.The radiographic hallmarks include shortened and horizontal ribs,squared scapulae and elevated clavicles with lateral kinking,normal spine and pelvis configuration, and shortening of the bonesof all four extremities, with extreme reduction of tibial bonelength.

Similarity:
Belongs to the protein kinase superfamily. NEK Ser/Thrprotein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.

SWISS:
Q96PY6

Gene ID:
4750

Database links:

Entrez Gene: 4750 Human

Entrez Gene: 3604 Mouse

Omim: 604588 Human

SwissProt: Q96PY6 Human

SwissProt: P51954 Mouse

Unigene: 481181 Human

Unigene: 486881 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (NEK1) Polyclonal Antibody, Unconjugated (SL7814R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.