Sarcosin contains 1 BTB (POZ) domain and is required for pseudopod elongation in transformed cells. Sarcosin mRNA is up-regulated by less than two folds in the heart in human patients with HCM.
Function:
Required for pseudopod elongation in transformed cells. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Subunit:
Interacts with NRAP (By similarity). Part of a complex that contains CUL3, RBX1 and KBTBD10.
Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, pseudopodium. Cell projection, ruffle. Note=Predominantly cytoplasmic but can co-localize with F-actin at the membrane ruffle-like structures at the tips of transformation-specific pseudopodia.
Post-translational modifications:
Ubiquitinated and probably targeted for proteasome-independent degradation.
Similarity:
Contains 1 BTB (POZ) domain.
Contains 5 Kelch repeats.
SWISS:
O60662
Gene ID:
10324
Database links:
Entrez Gene: 505794 Cow
Entrez Gene: 10324 Human
Entrez Gene: 228003 Mouse
Entrez Gene: 117537 Rat
SwissProt: O60662 Human
SwissProt: A2AUC9 Mouse
SwissProt: Q9ER30 Rat
Unigene: 50550 Human
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Sample:
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti- KBTBD10 (SL8044R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
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