Rabbit Anti-SRRM2 antibody | Sunlong Medical

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300 kDa nuclear matrix antigen; CWC21; KIAA0324; Ser/Arg-related nuclear matrix protein of 300 kDa; Serine/arginine repetitive matrix protein 2; Serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa; Splicing coactivator subunit S

Cat:

SL8067R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human SRRM2:101-230/2752

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SRRM2 Polyclonal Antibody, Unconjugated(SL8067R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The SRm160/300 splicing coactivator, which consists of the serine/arginine (SR)-related nuclear matrix protein and a nuclear matrix antigen, functions in splicing by promoting critical interactions between splicing factors bound to pre-mRNA. This splicing pathway involves five core small nuclear ribonucleoprotein particles (snRNPs) and the SR family proteins, which coordinately bind to pre-mRNA slicing enhancer elements, are required for accurate splice site recognition, and regulate alterative splicing patterns. The recognized splicing enhancer elements, known also as exonic enhancer splicing sequences, are short RNA sequences that are capable of activating weak splice sites in adjacent introns and contain specific binding sites for the serine/arginine (SR)-rich splicing factors. SRm160 and 300 antigens contain domains rich in SR motifs, but are distinctly different from the SR factors as they lack an RNA recognition motif and cannot directly induce RNA splicing. These proteins rather function as coactivators that stabilize the splicing complex and mediate the U1 snRNP-splicing pathway.

Function:
Involved in pre-mRNA splicing. May function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome. May do so by stabilizing the catalytic center or the position of the RNA substrate By similarity. Binds to RNA.

Subunit:
Component of the active spliceosome. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRP70, SNRPA1, SRRM1 and SRRM2. Identified in the spliceosome C complex.

Subcellular Location:
Nucleus speckle.

Tissue Specificity:
Expressed in liver, placenta, and white blood cells.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the CWC21 family.

SWISS:
Q9UQ35

Gene ID:
23524

Database links:

Entrez Gene: 23524 Human

Omim: 606032 Human

SwissProt: Q9UQ35 Human

Unigene: 433343 Human

Picture

Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SRRM2 Polyclonal Antibody, Unconjugated(SL8067R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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