Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.
Tissue specificity:Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.
Function:
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactivates RAB35, then activates RAB35 for its subsequent function at endosomes.
Subunit:
Interacts with RAB35 in the absence of nucleotide, but not in the presence of GTP. Interacts with clathrin heavy chain/CLTC and with AP2A2. Isoform 5 interacts with AP2B1.
Subcellular Location:
Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle.
Tissue Specificity:
Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells.
Post-translational modifications:
Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.
Similarity:
Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.
SWISS:
Q6P3S1
Gene ID:
163486
Database links:
Entrez Gene: 163486 Human
Omim: 613292 Human
SwissProt: Q6P3S1 Human
Unigene: 125056 Human
| Picture |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- DENND1B (SL8254R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 49 kD
|
|
|
