SESN2 is a member of the sestrin family of PA26-related proteins. The It may function in the regulation of cell growth and survival and also in cellular response to different stress conditions.
Function:
Involved in the reduction of peroxiredoxins.
Subcellular Location:
Cytoplasmic and Nuclear
Tissue Specificity:
Widely expressed.
Similarity:
Belongs to the sestrin family.
SWISS:
P58004
Gene ID:
83667
Database links:
Entrez Gene: 83667 Human
Entrez Gene: 230784 Mouse
Entrez Gene: 502988 Rat
Omim: 607767 Human
SwissProt: P58004 Human
SwissProt: P58043 Mouse
Unigene: 469543 Human
Unigene: 2728 Mouse
Unigene: 37672 Rat
| Picture |
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (SL8326R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (SL8326R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
|
|
