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Home > Product > Antibody > Rabbit Anti-EGR3 antibody
early growth response 3; early growth response gene 3; early growth response protein 3; early growth responsive 3; EGR-3; MGC138484; pilot; zinc finger protein pilot; EGR3_HUMAN.
Cat:
SL6448R
Species Reactivity：
Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human EGR3:201-300/387
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EGR3) Polyclonal Antibody, Unconjugated (SL6448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


EGR3 is a member of the early growth response transcription factor family of C2H2 zinc finger proteins (other members EGR1, EGR2 and EGR4). EGR proteins are immediate early proteins, expression of which is swiftly upregulated in response to a wide range of extracellular stimuli. EGR3 is also thought to be involved in development of muscle spindles, and is upregulated in several regions of the brain in response to stress or injury.

Function:
Probable transcription factor involved in muscle spindle development.

Subcellular Location:
Nuclear.

Similarity:
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
Q06889

Gene ID:
1960

Database links:

Entrez Gene: 1960 Human

Entrez Gene: 13655 Mouse

Entrez Gene: 25148 Rat

Omim: 602419 Human

SwissProt: Q06889 Human

SwissProt: P43300 Mouse

SwissProt: P43301 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EGR3) Polyclonal Antibody, Unconjugated (SL6448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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