Home>Product>Antibody> Rabbit Anti-Factor X antibody
Activated factor Xa heavy chain; Coagulation factor; Coagulation factor X; EC 3.4.21.6; F10 antibody FA10_HUMAN; Factor Xa; FX; FXA; OTTHUMP00000018735; Prothrombinase; Stuart factor; Stuart Prower factor; Stuart-Prower factor; Coagulation factor X; Facto
Cat:
SL9501R
Species Reactivity:
Mouse,Rat,(predicted: Human,)
Immunogen:
KLH conjugated synthetic peptide derived from human Activated factor Xa heavy chain:401-488/488
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Plasma (Mouse) Lysate at 40 ugPlasma (Rat) Lysate at 40 ugPrimary: Anti-Factor X (SL9501R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29/34/50 kDObserved band size: 29 kD
Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor X (Stuart Prower factor, FX, F10) is a vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. The mature form of Factor X (Factor X A) is generated by Factor IX A- or Factor VII A-mediated cleavage at the tripeptide sequence, Arg-Lys-Arg, to yield a disulfide linked dimer. Together with the cofactor Factor V A and Ca2+ on the surface of platelets or endothelial cells, Factor X A coordinates as part of the prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Mutations at the Factor X locus resulting in Factor X deficiencies can contribute to hemorrhagic diathesis.
Function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Subunit: The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5.
Subcellular Location: Plasma; synthesized in the liver
Tissue Specificity: Secreted
Post-translational modifications: The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
[PTM] N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
[PTM] The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway). The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
DISEASE: Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sample:
Plasma (Mouse) Lysate at 40 ug
Plasma (Rat) Lysate at 40 ug
Primary: Anti-Factor X (SL9501R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29/34/50 kD
Observed band size: 29 kD
Product Feedback Wall
Specific References (1) | SL9501R has been referenced in 1 publications.
[IF=4.307] Devin Cao. et al. Vascular Endothelial Cells Produce Coagulation Factors That Control Their Growth via Joint Protease-Activated Receptor and C5a Receptor 1 (CD88) Signaling. Am J Pathol. 2022 Feb;192:361 IF ; Mouse.