Rabbit Anti-RNF40 antibody | Sunlong Medical

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BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; BRE1B_HUMAN; E3 ubiquitin protein ligase BRE1B; E3 ubiquitin-protein ligase BRE1B; Rb associated protein; RBP 95; RBP95; Ring finger protein 40; RNF 40; Rnf40; STARING; 95 kDa retinoblastoma associated pr

Cat:

SL9170R

Species Reactivity：

Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human RNF40:901-1001/1001

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-RNF40 Polyclonal Antibody, Unconjugated(SL9170R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF40 (RING finger protein 40), also known as BRE1B, Staring or RBP95, is a 1,001 amino acid nuclear protein that contains one RING-type zinc finger. Expressed ubiquitously with highest expression in heart, testis and pancreas, RNF40 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B and Syntaxin 1. In addition, RNF40 forms a ubiquitin ligase complex with UBCH6 (an E2 enzyme) and together, these proteins play a crucial role in regulation of the histone code. Four isoforms of RNF40 exist due to alternative splicing events.

Function:
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes.

Subunit:
Homodimer. Component of the RNF20/40 complex at least composed of 2 copies of RNF20/BRE1A, 2 copies of RNF40/BRE1B and UBE2E1/UBCH6. Interacts with RB1 and WAC.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.

Similarity:
Belongs to the BRE1 family.
Contains 1 RING-type zinc finger.

SWISS:
O75150

Gene ID:
9810

Database links:

Entrez Gene: 9810 Human

Omim: 607700 Human

SwissProt: O75150 Human

Unigene: 65238 Human

Picture

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-RNF40 Polyclonal Antibody, Unconjugated(SL9170R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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