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Home > Product > Antibody > Rabbit Anti-RNF6 antibody
E3 ubiquitin-protein ligase RNF6; Ring finger protein (C3H2C3 type) 6; Ring finger protein 6; RING H2 protein; RING H2 protein RNF6; RNF 6; RNF6; RNF6_HUMAN; SPG2.
Cat:
SL9172R
Species Reactivity：
Rat,(predicted: Human,Mouse,Chicken,Dog,Cow,Horse,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human RNF6:551-650/685
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF6) Polyclonal Antibody, Unconjugated (SL9172R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product PDFs
Datasheet:


The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008].

Function:
E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.

Subunit:
Interacts with LIMK1 (By similarity). Interacts with AR.

Subcellular Location:
Nucleus. Cytoplasm. Cell projection, axon. Nucleus, PML body. Note=Localizes to the PML nuclear bodies in Sertoli cells.

Tissue Specificity:
Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.

DISEASE:
Esophageal cancer (ESCR) [MIM:133239]: A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. Note=The disease may be caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the RNF12 family.
Contains 1 RING-type zinc finger.

SWISS:
Q9Y252

Gene ID:
6049

Database links:

Entrez Gene: 6049 Human

Entrez Gene: 74132 Mouse

Entrez Gene: 304271 Rat

Omim: 604242 Human

SwissProt: Q9Y252 Human

SwissProt: Q9DBU5 Mouse

Unigene: 136885 Human

Unigene: 26696 Mouse

Unigene: 53030 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF6) Polyclonal Antibody, Unconjugated (SL9172R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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