The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. SH3RF2 (SH3 domain containing ring finger 2), also known as RNF158, is a 729 amino acid protein with one RING-type zinc finger domain and three SH3 domains. Via its RING-type zinc finger domain, SH3RF2 binds an E2 ubiquitin-conjugating enzyme. This suggests that SH3RF2 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation. Due to alternative splicing events, SH3RF2 is expressed as two different isoforms.
Function:
Inhibits PPP1CA phosphatase activity. May be a E3 ubiquitin-protein ligase (Potential). May play a role in cardiac function.
Subunit:
Interacts with FASLG and PPP1CA.
Tissue Specificity:
Heart (at protein level). Heart and testis. In the heart, present in the apex, left atrium, right atrium, left ventricle and right ventricle, but not in the aorta.
Similarity:
Belongs to the SH3RF family.
Contains 1 RING-type zinc finger.
Contains 3 SH3 domains.
SWISS:
Q8TEC5
Gene ID:
153769
Database links:
Entrez Gene: 153769 Human
Omim: 613377 Human
SwissProt: Q8TEC5 Human
Unigene: 443728 Human
| Picture |
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-RNF158 Polyclonal Antibody, Unconjugated(SL9230R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
|
|
|
