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E3 ubiquitin protein ligase RNF138; E3 ubiquitin-protein ligase RNF138; hNARF; HSD 4; HSD4; MGC8758; NARF; Nemo like kinase associated RING finger protein; Nemo-like kinase-associated RING finger protein; NLK associated RING finger protein; NLK-associated
Cat:
SL9254R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human RNF138:51-150/245
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF138) Polyclonal Antibody, Unconjugated (SL9254R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.

Function:
E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.

Subunit:
Interacts with NLK.

Post-translational modifications:
Auto-ubiquitinated.

Similarity:
Contains 1 RING-type zinc finger.

SWISS:
Q8WVD3

Gene ID:
51444

Database links:

Entrez Gene: 51444 Human

Entrez Gene: 56515 Mouse

Entrez Gene: 94196 Rat

SwissProt: Q8WVD3 Human

SwissProt: Q9CQE0 Mouse

SwissProt: Q99PD2 Rat

Unigene: 30488 Human

Unigene: 253542 Mouse

Unigene: 6814 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF138) Polyclonal Antibody, Unconjugated (SL9254R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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