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X chromosome; X-linked; Deubiquitinating enzyme FAF X; Deubiquitinating enzyme FAF-X; DFFRX; Fafl; Fam; Fat facets homolog; Fat facets in mammals; Fat facets protein related X linked; Fat facets protein related X-linked; Fat facets protein-related; hFAM;
Cat:
SL9266R
Species Reactivity：
Mouse,(predicted: Human,Rat,Chicken,Dog,Cow,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human USP9X:65-170/2570
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (USP9X) Polyclonal Antibody, Unconjugated (SL9266R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Function:
Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.

Subunit:
Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Widely expressed in embryonic and adult tissues.

Similarity:
Belongs to the peptidase C19 family.

SWISS:
Q93008

Gene ID:
8239

Database links:

Entrez Gene: 8239 Human

Entrez Gene: 22284 Mouse

Entrez Gene: 363445 Rat

Omim: 300072 Human

SwissProt: Q93008 Human

SwissProt: P70398 Mouse

Unigene: 77578 Human

Unigene: 242646 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (USP9X) Polyclonal Antibody, Unconjugated (SL9266R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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