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Home > Product > Antibody > Rabbit Anti-USP10 antibody
Deubiquitinating enzyme 10; Ubiquitin carboxyl terminal hydrolase 10; Ubiquitin carboxyl-terminal hydrolase 10; Ubiquitin specific peptidase 10; Ubiquitin specific processing protease 10; Ubiquitin specific protease 10; Ubiquitin thioesterase 10; Ubiquiti
Cat:
SL9267R
Species Reactivity：
Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human USP10:461-560/798
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (USP10) Polyclonal Antibody, Unconjugated (SL9267R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Datasheet:


Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013].

Function:
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.

Subunit:
Interacts with G3BP, which may regulate its function. Interacts with p53/TP53, SNX3 and CFTR.

Subcellular Location:
Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Ubiquitinated. Deubiquitinated by USP13.

Similarity:
Belongs to the peptidase C19 family. USP10 subfamily.

SWISS:
Q14694

Gene ID:
9100

Database links:

Entrez Gene: 9100 Human

Entrez Gene: 479625 Dog

Entrez Gene: 22224 Mouse

Entrez Gene: 307905 Rat

Omim: 609818 Human

SwissProt: Q14694 Human

SwissProt: P52479 Mouse

SwissProt: Q3KR59 Rat

Unigene: 19403 Dog

Unigene: 136778 Human

Unigene: 256910 Mouse

Unigene: 421337 Mouse

Unigene: 24862 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (USP10) Polyclonal Antibody, Unconjugated (SL9267R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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