Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH3 (membrane-associated ring finger (C3HC4) 3), also known as RNF173, is a 253 amino acid multi-pass membrane protein that localizes to cytoplasmic vesicles and early endosomes and contains one RING-CH-type zinc finger. Involved in the pathway of protein modification, MARCH3 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to be involved in endosomal trafficking events.
Function:
E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (By similarity).
Subunit:
Interacts with MARCH2 and STX6 (By similarity).
Subcellular Location:
Cytoplasmic vesicle membrane. Early endosome membrane.
Similarity:
Contains 1 RING-CH-type zinc finger.
SWISS:
Q86UD3
Gene ID:
115123
Database links:
Entrez Gene: 115123 Human
Entrez Gene: 64253 Mouse
Entrez Gene: 364878 Rat
Omim: 613333 Human
SwissProt: Q86UD3 Human
SwissProt: Q8BRX9 Mouse
SwissProt: Q5XIE5 Rat
Unigene: 132441 Human
Unigene: 312623 Mouse
Unigene: 203312 Rat
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Sample:
Eye (Mouse) Lysate at 40 ug
Primary: Anti- MARCH3 (SL9337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
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