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Home > Product > Antibody > Rabbit Anti-SMURF1 antibody
Smad ubiquitination regulatory factor 1; Smad ubiquitination regulatory factor 1; Smad specific E3 ubiquitin ligase 1; E3 ubiquitin-protein ligase SMURF1; hSMURF1; Smad specific E3 ubiquitin ligase 1; SMAD specific E3 ubiquitin protein ligase 1; Smad ubiq
Cat:
SL9391R
Species Reactivity：
Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human SMURF1:65-170/757
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMURF1) Polyclonal Antibody, Unconjugated (SL9391R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.

Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Similarity:
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains.

SWISS:
Q9HCE7

Gene ID:
75154

Database links:

Entrez Gene: 57154 Human

Entrez Gene: 75788 Mouse

Omim: 605568 Human

SwissProt: Q9HCE7 Human

SwissProt: Q9CUN6 Mouse

Unigene: 189329 Human

Unigene: 27735 Mouse



Picture

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMURF1) Polyclonal Antibody, Unconjugated (SL9391R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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