Rabbit Anti-KCTD5 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-KCTD5 antibody

BTB/POZ domain-containing protein KCTD5; FLJ20040; KCTD5; KCTD5_HUMAN; Potassium channel tetramerisation domain containing 5.

Cat:

SL9431R

Species Reactivity：

Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human KCTD5:101-200/234

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: Rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-KCTD5 Polyclonal Antibody, Unconjugated(SL9431R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. KCTD5 (potassium channel tetramerisation domain containing 5) is a 234 amino acid protein that localizes predominantly in the cytoplasm but translocates to the nucleus upon interaction with REP proteins. Existing as a homopentamer and consisting of one BTB (POZ) domain, KCTD5 associates with GRASP55, CUL-3 and ubiquitinated proteins. Interaction with CUL-3 suggests KCTD5 functions as a substrate adapter protein in some E3 ligase complexes.

Subunit:
Homopentamer. Interacts (via SLCterminus) with GRASP55/GORASP2. Interacts with CUL3 and with ubiquitinated proteins. Interacts with adeno-associated virus 2 (AASLV2) REP proteins.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Note=Predominantly cytoplasmic, translocated to the nucleus upon interaction with Rep proteins.

Similarity:
Contains 1 BTB (POZ) domain.

SWISS:
Q9NXV2

Gene ID:
54442

Database links:

Entrez Gene: 54442 Human

Omim: 611285 Human

SwissProt: Q9NXV2 Human

Unigene: 61960 Human

Picture

Tissue/cell: Rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-KCTD5 Polyclonal Antibody, Unconjugated(SL9431R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall