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Home > Product > Antibody > Rabbit Anti-ZNF521 antibody
Early hematopoietic zinc finger; Early hematopoietic zinc finger protein; EHZF; Evi3; LYST-interacting protein 3; MGC142182; Zfp521; Zinc finger protein 521; ZN521_HUMAN; Znf521.
Cat:
SL6978R
Species Reactivity：
Rat,(predicted: Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human EHZF/ZN521:851-950/1311
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-EHZF/ZN521 Polyclonal Antibody, Unconjugated(SL6978R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with ESF1, a transcription factor involved specification of B-cell lineage; this interaction preventing ESF1 to bind DNA and activate target genes.

Function:
Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with ESF1, a transcription factor involved specification of B-cell lineage; this interaction preventing ESF1 to bind DNA and activate target genes.

Subunit:
Interacts with ESF1. Interacts with SMAD1 and SMAD4.

Subcellular Location:
Nucleus.

Tissue Specificity:
Predominantly expressed in hematopoietic cells. Present in organs and tissues that contain stem and progenitor cells, myeloid and/or lymphoid: placenta, spleen, lymph nodes, thymus, bone marrow and fetal liver. Within the hematopoietic system, it is abundant in CD34(+) cells but undetectable in mature peripheral blood leukocytes, and its levels rapidly decrease during the differentiation of CD34(+) cells in response to hemopoietins.

DISEASE:
Note=A chromosomal aberration involving ZNF521 is found in acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with PAX5. The translocation generates the PAX5-ZNF521 oncogene consisting of the N-terminus part of PAX5 and the SLCterminus part of ZNF521.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 30 C2H2-type zinc fingers.

SWISS:
Q96K83

Gene ID:
25925

Database links:

Entrez Gene: 25925 Human

Omim: 610974 Human

SwissProt: Q96K83 Human

Unigene: 116935 Human



Picture

Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-EHZF/ZN521 Polyclonal Antibody, Unconjugated(SL6978R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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