Rabbit Anti-CXCR2 antibody | Sunlong Medical

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CXC-chemokine receptor 2; CD 182; CD182; CD182 antigen; CDw128b; Chemokine (CXC) receptor 2; CMKAR2; CXCR 2; CXC R2; CXC-R2; CXCR2_HUMAN; C-X-C chemokine receptor type 2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 rec

Cat:

SL4836R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human CXCR2:61-160/360<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human THP-1 cell lysates Lane 2: Human U937 cell lysates Lane 3: Human HL60 cell lysates Lane 4: Human HepG2 cell lysates Primary: Anti-CXCR2 (SL4836R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDaSample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ugLane 2: U937 (Human) Cell Lysate at 30 ugPrimary: Anti-CXCR2 (SL4836R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45/55 kDObserved band size: 59 kDBlank control: Hela. Primary Antibody (green line): Rabbit Anti-CXCR2 (SL4836R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (SLCX-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009].

Function:
Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Post-translational modifications:
Phosphorylated upon ligand binding; which is required for desensitization.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P25025

Gene ID:
3579

Database links:

Entrez Gene: 3579 Human

Entrez Gene: 12765 Mouse

Entrez Gene: 29385 Rat

Omim: 146928 Human

SwissProt: P25025 Human

SwissProt: P35343 Mouse

SwissProt: P35407 Rat

Unigene: 846 Human

Unigene: 234466 Mouse

Unigene: 90347 Rat

Picture

Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 2: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-CXCR2 (SL4836R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/55 kD
Observed band size: 59 kD

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-CXCR2 (SL4836R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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